Former Barcelona and AC Milan star to come out of retirement aged 39 for Colombian side Santa Fe.
The legendary Brazilian hung up his boots four years ago after a short spell with Fluminense.
He also featured for Barcelona, AC Milan, Paris Saint-Germain and Flamengo over 17 years, with 97 caps for the Selecao.
The club have announced he will feature in a friendly on October 17 at the Estadio El Campin in Bogota, with the opposition to be decided.
Ronaldinho has been working for the Brazilian government as an ambassador for tourism.
“I hope to continue to speak positively about our country wherever I go, inviting people to come here. It makes me very proud,” he said this month.